Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization

As β-glucosidases represent the major bottleneck for industrial degradation of plant biomass, great efforts are being devoted to discover both novel and robust versions these enzymes, as well develop efficient inexpensive ways produce them. In this work, raw glycerol from chemical production biodiesel was tested carbon source fungus Talaromyces amestolkiae with aim producing enzyme β-glucosidase-enriched cocktails. Approximately 11 U/mL β-glucosidase detected in cultures, constituting cellulolytic activity. Proteomic analysis showed BGL-3 most abundant protein main β-glucosidase. This crude successfully used supplement a basal commercial cocktail (Celluclast 1.5 L) saccharification pretreated wheat straw, corroborating that even hardly exploitable wastes, such glycerol, can be secondary materials valuable enzymatic preparations framework circular economy.